Antigen

Antigen Class

Histone

Antigen

H3K27me2

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

Embryonic stem cells

strain

C57BL/6

chip antibody

H3K27me2 (Cell Signaling, Rabbit mAb, D18C8)

genotype

EZH2 rescue

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

NEBNext Ultra protocol (NEB, E7370S)

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

35981138

Reads aligned (%)

97.5

Duplicates removed (%)

41.5

Number of peaks

481 (qval < 1E-05)

mm9

Number of total reads

35981138

Reads aligned (%)

97.3

Duplicates removed (%)

41.6

Number of peaks

528 (qval < 1E-05)