Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EZH2

Cell type

Cell type Class
Pluripotent stem cell
Cell type
iPS cells
NA
NA

Attributes by original data submitter

Sample

source_name
hiPSC
line name
UtE-iPS-11
ip antibody
EZH2
antibody source
in-house
antibody lot
NA

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were lysed in ChIP buffer (50 mM Tris-HCl pH 7.9, 150 mM NaCl, 1% Triron X-100, 0.5% NP-40, 5 mM EDTA pH 8.0, 1 mM PMSF and protease inhibitor) and chromatin was sonicated to ~200 bp with a Diagenode Bioruptor Libraries were prepared according to manufacturer’s instructions (Illumina). Immunoprecipitated DNA was first end-repaired using End-It Repair Kit (Epicenter), tailed with an A using Klenow exo minus (NEB M0212), and ligated to custom adapters with LigaFast (Promega, city, state #M8225). Fragments of 300±50 bp were size-selected and subjected to ligation-mediated PCR amplification (LM-PCR), using Phusion DNA polymerase (NEB M0530).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
38194961
Reads aligned (%)
72.1
Duplicates removed (%)
11.2
Number of peaks
1587 (qval < 1E-05)

hg38

Number of total reads
38194961
Reads aligned (%)
73.0
Duplicates removed (%)
10.6
Number of peaks
1540 (qval < 1E-05)

Base call quality data from DBCLS SRA