Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K36me3

Cell type

Cell type Class
Bone
Cell type
U2OS
Tissue
bone
Lineage
mesoderm
Description
osteosarcoma from the tibia of a 15 year old, J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma, viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses, mycoplasma contamination was detected and eliminated in 1972, (PMID: 6081590)

Attributes by original data submitter

Sample

source_name
U2OS cells
cell type
U2OS Cells
genotype/variation
shCTRL
chip antibody
H3K36me3, Abcam ab9050, Lot GR59748-1

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For RNA seq, total RNA was extracted using QiaGen RNAplus kit. ChIP-seq were performed following the protocol: Using ChIP-seq technology to generate high-resolution profiles of histone modifications. O'Geen H, Echipare L, Farnham PJ. Methods Mol Biol. 2011;791:265-86. doi: 10.1007/978-1-61779-316-5_20. Library was contracted by the MD Anderson DNA sequencing Core facility following standard protocol.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
76227792
Reads aligned (%)
93.0
Duplicates removed (%)
17.0
Number of peaks
1128 (qval < 1E-05)

hg19

Number of total reads
76227792
Reads aligned (%)
92.5
Duplicates removed (%)
18.2
Number of peaks
1126 (qval < 1E-05)

Base call quality data from DBCLS SRA