GSM2775004: ChIP RIS H3K4me1 Rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K4me1
Cell type
Cell type Class
Lung
Cell type
IMR-90
Primary Tissue
Lung
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
IMR90
vector 1
ER:HRAS(G12V)
4oht
Yes
chip antibody
H3K4me1 (Clone CMA302; in house; Kimura et al., 2008; also available from MBL life sciences, CAT MABI0302)
passage
23-28
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Fixed cells were lysed and sonicated to generate 200-400 bp chromatin fragments before immunoprecipitation Libraries were prepared using the NEBnext ultra II DNA-library prep kit for Illumina (E7645) according to manufacturers instructions except that size selection was performed during the final bead purification step using SPRIselect beads (B23319, Beckman Coulter)