Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
Isogenic HEK293 Cells
cell line
Human Embryonal Kidney Cells
chip antibody
IgG (Santa Cruz, sc-2027, lot # A2412)
stably expressing
none
treatment
dexamethasone
concentration
100 nM
time
1 h

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and GR-DNA complexes were isolated with 1 µg of antibody. ChIP-Seq libraries were prepared for sequencing using standard Illumina protocols, as utilized at the Gene Core in EMBL, Heidelberg, Germany

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
206904662
Reads aligned (%)
93.9
Duplicates removed (%)
47.0
Number of peaks
3403 (qval < 1E-05)

hg19

Number of total reads
206904662
Reads aligned (%)
93.4
Duplicates removed (%)
47.9
Number of peaks
2886 (qval < 1E-05)

Base call quality data from DBCLS SRA