GSM1176706: 3KR dex SUMO 2 3 rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
SUMO2
Cell type
Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
Isogenic HEK293 Cells
cell line
Human Embryonal Kidney Cells
chip antibody
SUMO-2/3 (MBL International, M114-3, lot # 23)
stably expressing
SUMOylation-defective GR
treatment
dexamethasone
concentration
100 nM
time
1 h
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and GR-DNA complexes were isolated with 1 µg of antibody. ChIP-Seq libraries were prepared for sequencing using standard Illumina protocols, as utilized at the Gene Core in EMBL, Heidelberg, Germany