Antigen

Antigen Class

TFs and others

Antigen

SUMO2

Cell type

Cell type Class

Kidney

Cell type

293

Primary Tissue

Kidney

Tissue Diagnosis

Normal

Sample

source_name

Isogenic HEK293 Cells

cell line

Human Embryonal Kidney Cells

chip antibody

SUMO-2/3 (MBL International, M114-3, lot # 23)

stably expressing

wild-type GR

treatment

dexamethasone

concentration

100 nM

time

1 h

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and GR-DNA complexes were isolated with 1 µg of antibody. ChIP-Seq libraries were prepared for sequencing using standard Illumina protocols, as utilized at the Gene Core in EMBL, Heidelberg, Germany

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

242686730

Reads aligned (%)

99.2

Duplicates removed (%)

41.8

Number of peaks

52421 (qval < 1E-05)

hg19

Number of total reads

242686730

Reads aligned (%)

98.6

Duplicates removed (%)

42.8

Number of peaks

52116 (qval < 1E-05)