Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NR3C1

Cell type

Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
Isogenic HEK293 Cells
cell line
Human Embryonal Kidney Cells
chip antibody
GR (Santa Cruz, sc-1003, lot # D1812)
stably expressing
wild-type GR
treatment
dexamethasone
concentration
100 nM
time
1 h

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and GR-DNA complexes were isolated with 1 µg of antibody. ChIP-Seq libraries were prepared for sequencing using standard Illumina protocols, as utilized at the Gene Core in EMBL, Heidelberg, Germany

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
109631313
Reads aligned (%)
71.3
Duplicates removed (%)
24.7
Number of peaks
22693 (qval < 1E-05)

hg38

Number of total reads
109631313
Reads aligned (%)
72.8
Duplicates removed (%)
23.6
Number of peaks
22993 (qval < 1E-05)

Base call quality data from DBCLS SRA