GSM1176683: IgG control ChIP-seq 4; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
Prdm4-EGFP_ESCs_B6_mIgG_ChIP
strain background
129/Sv
cell line
CCE
cell type
mouse embryonic stem cells
genotype/variation
expressing Prdm4-EGFP
chip antibody
normal mouse IgG
chip antibody vendor
Santa Cruz
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were precleared with Protein G Dynabeads. Complexes were then isolated with 6µg anti-GFP monoclonal antibody (Invitrogen A11120, clone 3E6, IgG2a) or control Mouse IgG (Santa Cruz sc-2025, Mouse IgG) linked to Protein G Dynabeads. Sequencing libraries were then prepared using the Illumina ChIP-seq DNA Sample Prep Kit. Libraries were size selected at ~200bp and four libraries were multiplexed per lane on a HiSeq 2000.