Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Digestive tract
Cell type
Intestinal organoids
NA
NA

Attributes by original data submitter

Sample

source_name
intestinal epithelial organoids
tissue origin
organoids cultivated from patient-derived normal colon mucosa
passages
3-5 passages
antibody
Histone H3K27ac (Active Motif Cat# 39133, Lot# 31814008)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
2 week old intestinal epithelial organoids (passage 3-5) were pooled (n=12) and snap from prior to processing for chromatin immunoprecipitation and sequencing according to Mack SC et al Nature 2014 Feb 27;506(7489):445-50 Rubicon Genomics ThruPlex DNA-Seq 48S with single indices

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
45773729
Reads aligned (%)
98.5
Duplicates removed (%)
9.9
Number of peaks
34344 (qval < 1E-05)

hg19

Number of total reads
45773729
Reads aligned (%)
98.0
Duplicates removed (%)
10.7
Number of peaks
34254 (qval < 1E-05)

Base call quality data from DBCLS SRA