Antigen

Antigen Class

Histone

Antigen

H3K4me1

Cell type

Cell type Class

Prostate

Cell type

LNCAP

Primary Tissue

Prostate

Tissue Diagnosis

Carcinoma

Sample

source_name

LNAR_H3K4me1

cell line

LNAR'

chip antibody

Abcam ab8895

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP protocol outlined in methods of paper; Tumors harvested and flash frozen in liquid nitrogen, RNA was extracted via lysing in MP Biomedical Lysing Matrix tubes in homogenizer, then Trizol extraction, followed by cleanup using Qiagen RNEasy columns ChIP-seq and RNA-seq libraries prepared using standard KAPA Hyper Library and Illumina protocols

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

37907136

Reads aligned (%)

89.3

Duplicates removed (%)

0.5

Number of peaks

538 (qval < 1E-05)

hg19

Number of total reads

37907136

Reads aligned (%)

88.8

Duplicates removed (%)

1.0

Number of peaks

562 (qval < 1E-05)