SRX3159206
GSM2771563: HMB Input; Mus musculus; ChIP-Seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
Pro-B cells
NA
NA

Attributes by original data submitter

Sample

source_name
Lin- bone marrow
strain
C57BL/6
cell type
HOXA9/MEIS1-transformed proB lymphoblasts (HMB cells)
viral transduction
Hoxa9/Meis1
days in culture
25-31
culture condition
B cell (5ng/mL IL7, 5ng/mL Flt3L)
chip antibody
None
tissue
Lin- bone marrow

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were collected from sonicated cells. Chromatin fragments were isolated with antibody, RNase treated and column purified. Libraries were generated using Illumina ChIP-seq Library Preparation Kit

Sequencing Platform

instrument_model
Illumina HiSeq 2000

Where can I get the processing logs?

Read processing pipeline log

mm10

Number of total reads
65241591
Reads aligned (%)
98.5
Duplicates removed (%)
33.2
Number of peaks
555 (qval < 1E-05)

mm9

Number of total reads
65241591
Reads aligned (%)
98.2
Duplicates removed (%)
33.2
Number of peaks
639 (qval < 1E-05)

Base call quality data from DBCLS SRA