SRX3159197
GSM2771554: HMM Input; Mus musculus; ChIP-Seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
Myeloblasts
NA
NA

Attributes by original data submitter

Sample

source_name
Lin- bone marrow
strain
C57BL/6
cell type
HOXA9/MEIS1-transformed myeloblasts (HMM cells)
viral transduction
Hoxa9/Meis1
days in culture
25-31
culture condition
Myeloid (10ng/mL IL3) + 100nM 4OHT
chip antibody
None
tissue
Lin- bone marrow

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were collected from sonicated cells. Chromatin fragments were isolated with antibody, RNase treated and column purified. Libraries were generated using Illumina ChIP-seq Library Preparation Kit

Sequencing Platform

instrument_model
Illumina HiSeq 2000

Where can I get the processing logs?

Read processing pipeline log

mm10

Number of total reads
39562968
Reads aligned (%)
95.9
Duplicates removed (%)
14.0
Number of peaks
489 (qval < 1E-05)

mm9

Number of total reads
39562968
Reads aligned (%)
95.7
Duplicates removed (%)
13.9
Number of peaks
537 (qval < 1E-05)

Base call quality data from DBCLS SRA