Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Blood

Cell type

Myeloid progenitors

NA

NA

Sample

source_name

Lin- bone marrow

strain

C57BL/6

cell type

untransformed myeloid progenitors (MP)

viral transduction

None

days in culture

7

culture condition

Stem cell (10ng/mL IL3, 10ng/mL IL6 100mg/mL SCF)

chip antibody

H3K27ac (Abcam ab4729)

tissue

Lin- bone marrow

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were collected from sonicated cells. Chromatin fragments were isolated with antibody, RNase treated and column purified. Libraries were generated using Illumina ChIP-seq Library Preparation Kit

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

43006757

Reads aligned (%)

87.7

Duplicates removed (%)

22.2

Number of peaks

27779 (qval < 1E-05)

mm9

Number of total reads

43006757

Reads aligned (%)

87.6

Duplicates removed (%)

22.3

Number of peaks

27818 (qval < 1E-05)