Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
DNMT1

Cell type

Cell type Class
Lung
Cell type
HBEC3-KT
Tissue
Lung, Bronchial
Cell Type
Epithelial
Disease
Normal

Attributes by original data submitter

Sample

source_name
Human bronchial epithelial cells
agent
CSC
cell line
HBEC-3KT
treatment time
10 Days
chip antibody
DNMT1 (Sigma-Aldrich, D4692)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were constructed according to the Illumina’s TruSeq DNA Sample Preparation v2 Kit protocol and sequenced on an Illumina HiSeq 2500 for 50 cycles

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
35156375
Reads aligned (%)
90.9
Duplicates removed (%)
6.9
Number of peaks
1200 (qval < 1E-05)

hg38

Number of total reads
35156375
Reads aligned (%)
93.5
Duplicates removed (%)
5.3
Number of peaks
1256 (qval < 1E-05)

Base call quality data from DBCLS SRA