GSM2766558: CSC10DDNMT1 R1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
DNMT1
Cell type
Cell type Class
Lung
Cell type
HBEC3-KT
Tissue
Lung, Bronchial
Cell Type
Epithelial
Disease
Normal
Attributes by original data submitter
Sample
source_name
Human bronchial epithelial cells
agent
CSC
cell line
HBEC-3KT
treatment time
10 Days
chip antibody
DNMT1 (Sigma-Aldrich, D4692)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were constructed according to the Illumina’s TruSeq DNA Sample Preparation v2 Kit protocol and sequenced on an Illumina HiSeq 2500 for 50 cycles