Curated Sample Data


Genome
dm3
Antigen Class
Input control
Antigen
Input control
Cell type Class
Embryo
Cell type
0-2h embryos

Cell type information


NA
NA

Attributes by Original Data Submitter


source_name
Early Embryo
stock
w1118
developmental stage
early 0-2 hpf embryos
chip antibody
none

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Embryos were collected in peach juice plates at 0-2 hours after fertilization (for details see Materials and Methods section in Pérez-Montero et al., 2013). ChIP-seq, library construction, cluster generation and Sequencing analysis were performed using the Illumina HiSeq2000, sequencing type SR50, following manufacture's protocols (www.illumina.com). In brief, libraries were prepared using Illumina's ChIP-Seq Sample Prep Kit from 3.5 ng of ChIP/Input DNA. Samples were size selected to ̴300-350 bp using DNA magnetic beads. Adaptor-modified DNA fragments were subjected to limited PCR amplification (18 cycles). Using Illumina's Cluster Generation Kit, libraries were subject to cluster generation for subsequent sequencing.

Platform Information


instrument_model
Illumina Genome Analyzer IIx

External Database Query

Logs in read processing pipeline


Number of total reads
30802466
Reads aligned (%)
28.1
Duplicates removed (%)
29.2
Number of peaks
2290 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA