Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me2

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
C57BL/6.mESC
genotype
inducible MT EED rescue 24hr
chip antibody
H3K27Me2, Cell Signaling, #9728

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed with 1% Formaldehyde. Nuclei was isolated and chromatin was fragmented. Chromatin immunoprecipitation (ChIP) was performed and the libraries was prepared using the eluted DNA from ChIP. Eluted DNA from ChIP was repaired to generate blunt 5' P ends using 'EndIT DNA End Repair kit' (Epicentre), 3' A overhang was generated using 'Klenow Fragment (3´→ 5´ exo-)' (NEB). Illumina barcodes were ligated using 'Rapid T4 DNA Ligase' (Enzymatics). Next libraries were size selected using 'Ampure beads' (Beckman Coulter) . After around 13 cycles of amplification, libraries were cleaned, quantified and pooled.

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
30026538
Reads aligned (%)
40.4
Duplicates removed (%)
18.4
Number of peaks
3044 (qval < 1E-05)

mm9

Number of total reads
30026538
Reads aligned (%)
40.4
Duplicates removed (%)
18.5
Number of peaks
3054 (qval < 1E-05)

Base call quality data from DBCLS SRA