Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
Mouse Embryonic Stem Cells
strain/background
C57BL/6
cell type
Differentiated cells sorted for Eomes (positive)
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cell lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries for sequencing were prepared with MicroPlex v2 kit (Diagenode) using 10ng of Chromatin without size selection from total input chromatin and immunoprecipitated DNA. Libraries were subjected to paired-end read analysis on an Illumina HiSeq 2500.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
55382103
Reads aligned (%)
97.8
Duplicates removed (%)
12.0
Number of peaks
285 (qval < 1E-05)

mm9

Number of total reads
55382103
Reads aligned (%)
97.7
Duplicates removed (%)
12.6
Number of peaks
288 (qval < 1E-05)

Base call quality data from DBCLS SRA