Antigen

Antigen Class

TFs and others

Antigen

GFI1

Cell type

Cell type Class

Blood

Cell type

Kasumi-1

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

Kasumi-1(leukemic)

cell line

Kasumi-1

cell type

Acute Myeloid Leukemia

chip antibody

antibody GFI1 (Santa Cruz, N-20, polyclonal, Lot H3012 )

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin was fixed in 1% formaldehyde for 10 minutes, sheared to 200-700bp using a Bioruptor (Diagenode), immunoprecipitated using the indicated antisera, purified and sequenced using Illumina Genome Analyzers as recommended by the manufacturer.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

40670376

Reads aligned (%)

98.4

Duplicates removed (%)

54.9

Number of peaks

8560 (qval < 1E-05)

hg19

Number of total reads

40670376

Reads aligned (%)

97.9

Duplicates removed (%)

55.6

Number of peaks

8508 (qval < 1E-05)