Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
GFI1

Cell type

Cell type Class
Blood
Cell type
Kasumi-1
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
Kasumi-1(leukemic)
cell line
Kasumi-1
cell type
Acute Myeloid Leukemia
chip antibody
antibody GFI1 (Santa Cruz, N-20, polyclonal, Lot H3012 )

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was fixed in 1% formaldehyde for 10 minutes, sheared to 200-700bp using a Bioruptor (Diagenode), immunoprecipitated using the indicated antisera, purified and sequenced using Illumina Genome Analyzers as recommended by the manufacturer.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
40670376
Reads aligned (%)
43.5
Duplicates removed (%)
55.6
Number of peaks
8508 (qval < 1E-05)

hg38

Number of total reads
40670376
Reads aligned (%)
44.4
Duplicates removed (%)
54.9
Number of peaks
8560 (qval < 1E-05)

Base call quality data from DBCLS SRA