GSM2759024: HeLa U1 AMO 8hr IgG ChIP; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
Human Carcinoma Cells
cell line
HeLa
strain
PV
transfection
U1
chip antibody
Normal mouse IgG (Santa Cruz Biotechnology sc-2025)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were harvested and then crosslinked with 1% Formaldehyde. Nuclei were chemically isolated and then lysed, then sheared using a sonicator. The resulting sample was cleared using dynabeads, and polII-DNA complexes were isolated with the antibody. Libraries were prepared according to instructions accompanying the TruSeq ChIP sample preparation kit from Illumina (# IP-202-1012). Briefly, the overhanging DNA ends were blunted using exonuclease, and then the 3' end was adenylated to prevent improper ligation. The adapters were then ligated, and the library was amplified.