GSM2757306: Input DNA (p19 control); Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Pluripotent stem cell
Cell type
P19
Tissue
Embryo
Disease
Teratocarcinoma; Embryonal Carcinoma
Attributes by original data submitter
Sample
source_name
mouse embryonic carcinoma cells
cell line
p19 cells
stable integrate
pBI-EGFP-FLAG-mCtcfl and pTA-N
treatment
Cells were kept in DMEM supplemented with 2 μg/ml Doxycycline, for induction the cells were grown for 48h in DMEM without Doxycycline
antibody
NA
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was isolated and sheared using BioRuptor (Diagenode). ChIPs were performed using a One-Day Protocol as described by Nelson et al. (2006 / Nature Protocols / DOI: 10.1038/nprot.2006.27 Sequencing libraries were prepared from 10 ng of immunoprecipitated DNA with the Illumina ChIPSeq DNA Sample Prep Kit according to Illuminas’ instructions