Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Uterus

Cell type

HeLa

Primary Tissue

Cervix

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

HeLa, CSC, H3K4me3

cell type

HeLa cells transfected with ZsGreen-cODCdegron

chip antibody

H3K4me3 (diagenode, C15410003-50)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the TruSeq ChIP Sample Prep Kit (Part# 15023092 Rev. B).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

39933548

Reads aligned (%)

98.4

Duplicates removed (%)

7.0

Number of peaks

17320 (qval < 1E-05)

hg19

Number of total reads

39933548

Reads aligned (%)

97.5

Duplicates removed (%)

8.6

Number of peaks

16922 (qval < 1E-05)