Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
HeLa, nonCSC, H3K4me3
cell type
HeLa cells transfected with ZsGreen-cODCdegron
chip antibody
H3K4me3 (diagenode, C15410003-50)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the TruSeq ChIP Sample Prep Kit (Part# 15023092 Rev. B).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
38678203
Reads aligned (%)
97.4
Duplicates removed (%)
11.0
Number of peaks
23125 (qval < 1E-05)

hg19

Number of total reads
38678203
Reads aligned (%)
96.6
Duplicates removed (%)
12.4
Number of peaks
22541 (qval < 1E-05)

Base call quality data from DBCLS SRA