Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Muscle
Cell type
Muscle satellite cells
NA
NA

Attributes by original data submitter

Sample

source_name
Skeletal muscle stem cells (satellite cells)
fixation
T3
chip antibodies
input sample
strain
C57BL/6

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Genomic DNA was extracted after cell lysis and immunoprecipitated by Diagenode ChIP-seq grade antibodies using the Diagenode iDeal Histones ChIP-seq kit Libraries were prepared with the NEBNext ultra 2 kit (NEB E7645L). Library pools were separated on an agarose gel and DNA of a 100-280 bp size-range was selected for sequencing on an Illumina HiSEQ 4000 as paired-end 75-bp reads.

Sequencing Platform

instrument_model
Illumina HiSeq 4000

mm10

Number of total reads
17190899
Reads aligned (%)
97.3
Duplicates removed (%)
14.9
Number of peaks
262 (qval < 1E-05)

mm9

Number of total reads
17190899
Reads aligned (%)
97.1
Duplicates removed (%)
15.0
Number of peaks
293 (qval < 1E-05)

Base call quality data from DBCLS SRA