Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Pluripotent stem cell

Cell type

iPSC derived hepatocyte-like cells

NA

NA

Sample

source_name

human induced hepatocyte-like cells

tissue

human induced hepatocyte-like cells

cell type

hepatocyte-like cells

chip antibody

H3K27ac(abcam, ab4729)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Briefly, DNA was end-repaired using End-Repair Enzyme mix(Epicentre #ER0720) and dATP was added to 3’ends of DNA using Klenow fragment(NEB).After ligation of adaptors from Illumina Truseq DNA sample prep kit by T4 DNA ligase (NEB).DNA was PCR amplified with Illumina universal primers, library fragments near ~300bp were isolated from agarose gel and massive parallel sequencing was performed using Illumina HiSeq2000 sequencer.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

24147889

Reads aligned (%)

99.1

Duplicates removed (%)

3.5

Number of peaks

22497 (qval < 1E-05)

hg19

Number of total reads

24147889

Reads aligned (%)

98.5

Duplicates removed (%)

4.1

Number of peaks

22371 (qval < 1E-05)