Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Placenta

Cell type

Umbilical Cord

MeSH Description

The flexible rope-like structure that connects a developing FETUS to the PLACENTA in mammals. The cord contains blood vessels which carry oxygen and nutrients from the mother to the fetus and waste products away from the fetus.

Sample

source_name

umbilical cord

tissue

Fibroblast

cell type

umbilical cord

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Briefly, DNA was end-repaired using End-Repair Enzyme mix(Epicentre #ER0720) and dATP was added to 3’ends of DNA using Klenow fragment(NEB).After ligation of adaptors from Illumina Truseq DNA sample prep kit by T4 DNA ligase (NEB).DNA was PCR amplified with Illumina universal primers, library fragments near ~300bp were isolated from agarose gel and massive parallel sequencing was performed using Illumina HiSeq2000 sequencer.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

38312544

Reads aligned (%)

98.2

Duplicates removed (%)

4.3

Number of peaks

1585 (qval < 1E-05)

hg19

Number of total reads

38312544

Reads aligned (%)

96.8

Duplicates removed (%)

5.9

Number of peaks

947 (qval < 1E-05)