GSM2753126: mm input spreb tag.1; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Blood
Cell type
Pre-B cells
NA
NA
Attributes by original data submitter
Sample
source_name
SpreB – input tagmentation
cell type
small Pre-B
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin from flow sorted small pre-B cells (20×10^6 or 2×10^6 for lowdna samples) was used for each ChIP experiment with anti-IRF4 (M-17, Santa Cruz Biotechnogy, sc-6059X, lot#J2015) antibody. DNA libraries were prepared from the sheared chromatin (200–600 bp). Libraries were sequenced on the Illumina Hiseq2500.