Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
Pre-B cells
NA
NA

Attributes by original data submitter

Sample

source_name
SpreB – input tagmentation
cell type
small Pre-B

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin from flow sorted small pre-B cells (20×10^6 or 2×10^6 for lowdna samples) was used for each ChIP experiment with anti-IRF4 (M-17, Santa Cruz Biotechnogy, sc-6059X, lot#J2015) antibody. DNA libraries were prepared from the sheared chromatin (200–600 bp). Libraries were sequenced on the Illumina Hiseq2500.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
55525465
Reads aligned (%)
92.8
Duplicates removed (%)
10.6
Number of peaks
401 (qval < 1E-05)

mm9

Number of total reads
55525465
Reads aligned (%)
92.7
Duplicates removed (%)
10.6
Number of peaks
399 (qval < 1E-05)

Base call quality data from DBCLS SRA