GSM2752586: jc2914 MCF7 ER AZD2014; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
ESR1
Cell type
Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
ERa AZD2014 treatment
cell type
Breast cancer
passages
p20
cell line
MCF7
chip antibody
ERa sc-543
agent
Treatment with AZD201
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked for 10min using 1% formaldehyde. After sonication, chromatin immunoprecipitation was performed using a polyclonal ERa antibody over night. After decrosslinking at 65 C over night followed by RNase A and proteinase K treatment, immunoprecipitated DNA was purified by phenol-chloroform extraction. DNA was prepared for Illumina sequencing on the HiSeq 2500 using the ThruPLEX DNA-seq kit according to the manufacturer's instructions.