GSM2752584: jc2912 MCF7 ER Veh; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
TFs and others
Cell type Class
Site of Extraction
Attributes by original data submitter
Sequenced DNA Library
Cells were crosslinked for 10min using 1% formaldehyde. After sonication, chromatin immunoprecipitation was performed using a polyclonal ERa antibody over night. After decrosslinking at 65 C over night followed by RNase A and proteinase K treatment, immunoprecipitated DNA was purified by phenol-chloroform extraction. DNA was prepared for Illumina sequencing on the HiSeq 2500 using the ThruPLEX DNA-seq kit according to the manufacturer's instructions.