GSM1171653: Hela Input 20G2C12 12278; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
Hela_Input_20G2C12
cell line
HeLa
chip antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed for 10min at RT in 1% formaldehyde, harvested using a cell scraper, washed once in ice-cold PBS, and resuspended in RIPA buffer with protease inhibitor. The sample was then sonicated using an 3.2mm microtip (QSonica Sonicator 4000) at 30s on 30s off intervals and 40% amplitude for 180min while in a -30C 3:1 isopropanol and water bath containing dry ice. Subsequent steps were performed as per the standard protocol. DNA was size-selected during library building to an average fragment size of 200bp. Libraries were sequenced using Illumina HiSeq 2000. Libraries were generated following Illumina's DNA Sample Prep Kit protocol