Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ESR1

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MCF7_ER_H2av_S2
cell line
MCF7
cell type
mammary epithelial cells
experiment number
SLX-8047
chip antibody
ER (sc-543, Santa Cruz)
control antibody
H2av (39715, ActiveMotif)
condition
none
spike-in cell line
S2
spike-in organism
drosophila melanogaster
replicate
4

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. Libraries were prepared according to the Thruplex DNA-seq Kit Manual

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg19

Number of total reads
17593888
Reads aligned (%)
56.7
Duplicates removed (%)
5.8
Number of peaks
7231 (qval < 1E-05)

hg38

Number of total reads
17593888
Reads aligned (%)
57.5
Duplicates removed (%)
4.9
Number of peaks
7200 (qval < 1E-05)

Base call quality data from DBCLS SRA