GSM2746549: SW480 16hr TNF-a input; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Digestive tract
Cell type
SW 480
Primary Tissue
unresolved
Tissue Diagnosis
unresolved
Attributes by original data submitter
Sample
source_name
SW480_SW480 16hr TNF-α input
cell line
SW480
cell type
Human Colon Cancer Cell Line
chip antibody
12.5 ng ml-1 TNF-α for 16 hr
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For GRO-seq analyses, nuclei were prepared and used for run-on reactions using BrUTP. Following nascent RNA enrichment with anti-BrdU agarose beads (SCBT), RNA was extracted using TRIzol LS reagent and used for library preparation. For ChIP, lysates from sonicated nuclei were prepared and protein-DNA complexes were IP'd with corresponding antibodies. For RNA-seq, total RNA was extracted using TRIzol LS reagent and strand-specific libraries were prepared from 1 mg of RNA ChIP-seq libraries were prepared according to Illumina's ChIP Truseq protocol. RNA-seq libraries were prepared following the dUTP second strand cDNA method using NEXTflex barcodes. GRO-seq libraries were prepared similarly. Please refer to the methods section for detailed protocols.