Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Tcf3

Cell type

Cell type Class
Blood
Cell type
Mast Cells
MeSH Description
Granulated cells that are found in almost all tissues, most abundantly in the skin and the gastrointestinal tract. Like the BASOPHILS, mast cells contain large amounts of HISTAMINE and HEPARIN. Unlike basophils, mast cells normally remain in the tissues and do not circulate in the blood. Mast cells, derived from the bone marrow stem cells, are regulated by the STEM CELL FACTOR.

Attributes by original data submitter

Sample

source_name
ChIPseq_E2A_mast
cell type
primary mast cells

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP assays were performed as previously described (Wilson NK et al., 2010 Cell Stem Cell) using polyclonal antibodies against CTCF (Upstate, 07-729), E2A (Santa Cruz, sc-763x), Erg (Santa Cruz, sc354x), FLI-1 (Abcam, ab15289-500), GATA2 (Santa Cruz, sc9008x), LMO2 (R&D, AF2726), Meis1 (Santa Cruz, sc-10599x), PU.1 (Santa Cruz, sc-352x), RUNX1 (Abcam, ab23980-100), TAL1 (Santa Cruz, sc12984x), Mitf (Cosmo Bio Co., BAM-73-107-EX), cFos (Santa Cruz, sc-253x). Each ChIPseq sample was amplified and sequenced using the Illumina Genome Analyzer IIx, following manufacturer's instructions.

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

mm10

Number of total reads
36422229
Reads aligned (%)
30.9
Duplicates removed (%)
26.7
Number of peaks
1072 (qval < 1E-05)

mm9

Number of total reads
36422229
Reads aligned (%)
30.9
Duplicates removed (%)
26.9
Number of peaks
1078 (qval < 1E-05)

Base call quality data from DBCLS SRA