Curated Sample Data


Genome
mm9
Antigen Class
TFs and others
Antigen
Tcf3
Cell type Class
Blood
Cell type
HPC-7

Cell type information


NA
NA

Attributes by Original Data Submitter


source_name
ChIPseq_E2A_HPC7
cell line
HPC7
cell type
ES-derived mutipotent haematopoietic progenitor cell line

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP assays were performed as previously described (Wilson NK et al., 2010 Cell Stem Cell) using polyclonal antibodies against CTCF (Upstate, 07-729), E2A (Santa Cruz, sc-763x), Erg (Santa Cruz, sc354x), FLI-1 (Abcam, ab15289-500), GATA2 (Santa Cruz, sc9008x), LMO2 (R&D, AF2726), Meis1 (Santa Cruz, sc-10599x), PU.1 (Santa Cruz, sc-352x), RUNX1 (Abcam, ab23980-100), TAL1 (Santa Cruz, sc12984x), Mitf (Cosmo Bio Co., BAM-73-107-EX), cFos (Santa Cruz, sc-253x). Each ChIPseq sample was amplified and sequenced using the Illumina Genome Analyzer IIx, following manufacturer's instructions.

Platform Information


instrument_model
Illumina Genome Analyzer IIx

External Database Query

Logs in read processing pipeline


Number of total reads
40328875
Reads aligned (%)
88.8
Duplicates removed (%)
14.1
Number of peaks
22108 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA