Antigen

Antigen Class

TFs and others

Antigen

Ctcf

Cell type

Cell type Class

Blood

Cell type

HPC-7

NA

NA

Sample

source_name

ChIPseq_Ctcf_HPC7

cell line

HPC7

cell type

ES-derived mutipotent haematopoietic progenitor cell line

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP assays were performed as previously described (Wilson NK et al., 2010 Cell Stem Cell) using polyclonal antibodies against CTCF (Upstate, 07-729), E2A (Santa Cruz, sc-763x), Erg (Santa Cruz, sc354x), FLI-1 (Abcam, ab15289-500), GATA2 (Santa Cruz, sc9008x), LMO2 (R&D, AF2726), Meis1 (Santa Cruz, sc-10599x), PU.1 (Santa Cruz, sc-352x), RUNX1 (Abcam, ab23980-100), TAL1 (Santa Cruz, sc12984x), Mitf (Cosmo Bio Co., BAM-73-107-EX), cFos (Santa Cruz, sc-253x). Each ChIPseq sample was amplified and sequenced using the Illumina Genome Analyzer IIx, following manufacturer's instructions.

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

mm10

Number of total reads

40085503

Reads aligned (%)

94.5

Duplicates removed (%)

40.2

Number of peaks

78505 (qval < 1E-05)

mm9

Number of total reads

40085503

Reads aligned (%)

94.3

Duplicates removed (%)

40.2

Number of peaks

78458 (qval < 1E-05)