Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ETV6

Cell type

Cell type Class
Blood
Cell type
REH
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Lymphocytic

Attributes by original data submitter

Sample

source_name
pre-B leukemia cell line, Positive IP/test condition
cell line
Reh (ATCC CRL-8286)
transduction
pCCL ETV6-HA
chip antibody
Anti-HA magnetic beads (Thermo Fisher Scientific)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and ETV6-HA/DNA complexes were isolated with Anti-HA magnetic beads. Protein/DNA complexes were eluted from the beads by competition with HA peptides prior to reverse-crosslinking and standard purification using phenol/chloroform/isoamyl alcohol The purified ChIP DNA was processed through TruSeq ChIP Sample Preparation Kit (Illumina) according to the manufacturer protocol

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
92095712
Reads aligned (%)
0.0
Duplicates removed (%)
3.5
Number of peaks
678 (qval < 1E-05)

hg19

Number of total reads
92095712
Reads aligned (%)
0.0
Duplicates removed (%)
3.5
Number of peaks
606 (qval < 1E-05)

Base call quality data from DBCLS SRA