Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Epidermis

Cell type

Keratinocytes

MeSH Description

Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers of the epidermis to the cornified (horny) layer of the skin. Successive stages of differentiation of the keratinocytes forming the epidermal layers are basal cell, spinous or prickle cell, and the granular cell.

Sample

source_name

HKC

chip antibody

non-immune IgG control

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

DNA was immunoprecipitated from 5 x 10e6 HKCs using 10 μl of CSL antibody per 10e6 cells, followed by quantification by fluorometry on a Qubit system (Invitrogen). A total of 10 ng of DNA was used for library preparation using NEBNext ChIP-Seq Library Prep Kit (Illumina) according to manufacturer’s recommendation.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

143569354

Reads aligned (%)

95.4

Duplicates removed (%)

6.3

Number of peaks

3613 (qval < 1E-05)

hg19

Number of total reads

143569354

Reads aligned (%)

94.6

Duplicates removed (%)

7.9

Number of peaks

1970 (qval < 1E-05)