Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Neural

Cell type

Corpus Striatum

MeSH Description

Striped GRAY MATTER and WHITE MATTER consisting of the NEOSTRIATUM and paleostriatum (GLOBUS PALLIDUS). It is located in front of and lateral to the THALAMUS in each cerebral hemisphere. The gray substance is made up of the CAUDATE NUCLEUS and the lentiform nucleus (the latter consisting of the GLOBUS PALLIDUS and PUTAMEN). The WHITE MATTER is the INTERNAL CAPSULE.

Sample

source_name

Mouse striatum

tissue type

striatum

age

4 months

htt genotype

Q111/+

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

striatal tissue was homogenized in glass dounce and fixed with formaldehyde for 10 min at room temperature. Chromatin was fragmented using MNase digestion followed by sonication. HTT-DNA complexes were isolated using Abcam EPR5526 antibody. library strategy: Modified ChIP-seq (see PMID 22025700)

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

21052506

Reads aligned (%)

93.5

Duplicates removed (%)

2.4

Number of peaks

147 (qval < 1E-05)

mm9

Number of total reads

21052506

Reads aligned (%)

93.5

Duplicates removed (%)

2.7

Number of peaks

157 (qval < 1E-05)