Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Adipocyte
Cell type
Subcutaneous adipocytes
NA
NA

Attributes by original data submitter

Sample

source_name
subcutaneous adipocytes
chip antibody
input

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP mature adipocyte cells were crosslinked with 1% formaldehyd for 10 min and sonicated to 200 bps. Genomic DNA was extracted using antibodies for H3K4me3, H3K9me3 and H3K27ac. The libraries for ChIP-Seq were preparated using the MicroPlex Library preparation kit (Diagenode) and purified with Agencourt AMPure XP beads (Beckman Coulter Life Sciences, Krefeld, Germany).

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg38

Number of total reads
35374743
Reads aligned (%)
99.0
Duplicates removed (%)
12.8
Number of peaks
1233 (qval < 1E-05)

hg19

Number of total reads
35374743
Reads aligned (%)
98.1
Duplicates removed (%)
14.3
Number of peaks
1177 (qval < 1E-05)

Base call quality data from DBCLS SRA