Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NCOA4

Cell type

Cell type Class
Blood
Cell type
Erythroblasts
MeSH Description
Immature, nucleated ERYTHROCYTES occupying the stage of ERYTHROPOIESIS that follows formation of ERYTHROID PRECURSOR CELLS and precedes formation of RETICULOCYTES. The normal series is called normoblasts. Cells called MEGALOBLASTS are a pathologic series of erythroblasts.

Attributes by original data submitter

Sample

source_name
erythroblasts
cell type
erythroblasts
chip antibody
Santa Cruz, sc-373739

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with specific antibodies. Genomic DNA was purified from BFU-Es sheared by sonication to 50-500 bp and processed with the Illumina ChIP-Seq DNA Sample Prep Kit (http://support.illumina.com/sequencing/sequencing_kits/chip-seq_dna_sample_prep_kit.html). Samples were sequenced using the Illumina HiSeq system

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
42039325
Reads aligned (%)
7.2
Duplicates removed (%)
51.3
Number of peaks
269 (qval < 1E-05)

hg38

Number of total reads
42039325
Reads aligned (%)
7.5
Duplicates removed (%)
50.0
Number of peaks
272 (qval < 1E-05)

Base call quality data from DBCLS SRA