Antigen

Antigen Class

TFs and others

Antigen

SMARCA2

Cell type

Cell type Class

Liver

Cell type

Hep G2

Primary Tissue

Liver

Tissue Diagnosis

Carcinoma Hepatocellular

Sample

source_name

HepG2_BRM CHIP-seq in shBRG1

cell line

HepG2

cell type

liver cancer cell line

tranduced with

shBrg1

chip antibody

BRM (D9E8B) XP® Rabbit mAb

chip antibody vendor

Cell Signaling

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were fixed in 0.3% formaldehyde for 30 minutes at 4 degrees prior to processing for ChIP using protocol from Raab et al 2015. Kapa DNA hyperpep kit

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg38

Number of total reads

24026983

Reads aligned (%)

92.2

Duplicates removed (%)

1.1

Number of peaks

722 (qval < 1E-05)

hg19

Number of total reads

24026983

Reads aligned (%)

91.4

Duplicates removed (%)

1.1

Number of peaks

658 (qval < 1E-05)