Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SMARCA4

Cell type

Cell type Class
Liver
Cell type
Hep G2
Primary Tissue
Liver
Tissue Diagnosis
Carcinoma Hepatocellular

Attributes by original data submitter

Sample

source_name
HepG2_BRG1 CHIP-seq in shBRG1
cell line
HepG2
cell type
liver cancer cell line
tranduced with
shBrg1
chip antibody
Anti-BRG1 antibody
chip antibody vendor
Abcam

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed in 0.3% formaldehyde for 30 minutes at 4 degrees prior to processing for ChIP using protocol from Raab et al 2015. Kapa DNA hyperpep kit

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg19

Number of total reads
16359329
Reads aligned (%)
87.4
Duplicates removed (%)
1.4
Number of peaks
576 (qval < 1E-05)

hg38

Number of total reads
16359329
Reads aligned (%)
88.1
Duplicates removed (%)
1.3
Number of peaks
542 (qval < 1E-05)

Base call quality data from DBCLS SRA