Curated Sample Data


Genome
dm3
Antigen Class
Input control
Antigen
Input control
Cell type Class
Embryo
Cell type
13-16h embryos

Cell type information


NA
NA

Attributes by Original Data Submitter


source_name
13-16h embryos
strain
w1118
developmental stage
13-16h embryos
chip antibody
rabbit IgG

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Embryos were homogenized and fixed in 1.8% formaldehyde at room temperature. After several washes, chromatin in lysis buffer was sonicated to a length range of 0.1kb-0.5kb. For each immunoprecipitation, sheared chromatin was precleared with Gammabind G agarose coated with BSA and incubated with pre-absorbed rabbit anti-Grh antibody, mouse anti-Grh antibody or rabbit IgG. Precipitated complexes were washed, eluted, and cross-links were reversed at 65°C. After Proteinase K treatment, DNA was purified using QIAprep Spin columns and recovered in 50 μl of elution buffer containing RNase A. DNA libraries were made using the Illumina ChIP-seq library kit

Platform Information


instrument_model
Illumina Genome Analyzer II

External Database Query

Logs in read processing pipeline


Number of total reads
48381350
Reads aligned (%)
59.7
Duplicates removed (%)
54.7
Number of peaks
6344 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA