Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
B cells
NA
NA

Attributes by original data submitter

Sample

source_name
B cells
cell type
Activated B cells
passages
Primary Cells
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chip-Sequencing were performed by ActiveMoitf society. For RNA sequencing, after sorting , RNA were extracted (NucleoSpin® RNA XS), measured (Qubit® 2.0 Thermo Fisher Scientific), and library were constructed using TruSeq® Stranded mRNA Sample Preparation Chip-Sequencing libraries and RNAsequencing librairies were performed according to illumina's instruction

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
36705181
Reads aligned (%)
97.9
Duplicates removed (%)
6.3
Number of peaks
1602 (qval < 1E-05)

hg19

Number of total reads
36705181
Reads aligned (%)
97.1
Duplicates removed (%)
7.6
Number of peaks
1446 (qval < 1E-05)

Base call quality data from DBCLS SRA