Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BACH2

Cell type

Cell type Class
Blood
Cell type
B cells
NA
NA

Attributes by original data submitter

Sample

source_name
B cells
cell type
Activated B cells
passages
Primary Cells
chip antibody
BACH2 (D3T3G)
transfection
siBACH2

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chip-Sequencing were performed by ActiveMoitf society. For RNA sequencing, after sorting , RNA were extracted (NucleoSpin® RNA XS), measured (Qubit® 2.0 Thermo Fisher Scientific), and library were constructed using TruSeq® Stranded mRNA Sample Preparation Chip-Sequencing libraries and RNAsequencing librairies were performed according to illumina's instruction

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
36927996
Reads aligned (%)
62.7
Duplicates removed (%)
34.3
Number of peaks
1778 (qval < 1E-05)

hg38

Number of total reads
36927996
Reads aligned (%)
64.4
Duplicates removed (%)
33.0
Number of peaks
1893 (qval < 1E-05)

Base call quality data from DBCLS SRA