GSM2738066: Input DNA in lytic cells; Homo sapiens; Human gammaherpesvirus 8; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Blood
Cell type
BCBL1
NA
NA
Attributes by original data submitter
Sample
source_name
TREx-F3H3-K-Rta BCBL-1 cells
pathogen
KSHV
tissue
peritoneal effusion
cell type
KSHV positive B cell lymphoma
passages
5~10
chip antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was sonicated to an average size of ~300 bp using a Diagenode Bioruptor. Chromatin DNA from 1 X 108 cells was used per immunoprecipitation assay with 10 µg of ZIC2 antibody ChIP-enriched and input DNA samples were used to generate Illumina-compatible libraries with the KAPA LTP Library Preparation Kit (Kapa Biosystems, KR0453) according to manufacturer’s recommendations.