Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BRD4

Cell type

Cell type Class
Breast
Cell type
MDA-MB-231
Primary Tissue
Breast
Site of Extraction
Effusion, Pleural
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
DMSO_BRD4, MDA-MB-231
cell line
MDA-MB-231 breast cancer cells
chip antibody
BRD4 (Bethyl Laboratories, A301-985A, lot A301-985A100-5)
treatment
DMSO

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cross-linked chromatin was sheared by sonication and immunoprecipitated with antibodies. DNA was purified by phenol/chloroform extraction and ethanol precipitation. NuGEN Ovation Ultralow Library Prep System kit

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
33739227
Reads aligned (%)
79.9
Duplicates removed (%)
17.9
Number of peaks
1206 (qval < 1E-05)

hg38

Number of total reads
33739227
Reads aligned (%)
83.6
Duplicates removed (%)
15.0
Number of peaks
1331 (qval < 1E-05)

Base call quality data from DBCLS SRA