Antigen

Antigen Class

TFs and others

Antigen

ESR1

Cell type

Cell type Class

Breast

Cell type

MCF-7

Primary Tissue

Breast

Site of Extraction

Pleura

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

MCF-7/c-Jun -Tet cells

cell line

MCF-7

cell type

ERalpha-positive breast cancer cells

chip antibody

ERalpha (H-20, Santa Cruz)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei, c-Jun and ERα complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

29278526

Reads aligned (%)

99.2

Duplicates removed (%)

9.1

Number of peaks

18486 (qval < 1E-05)

hg19

Number of total reads

29278526

Reads aligned (%)

98.5

Duplicates removed (%)

10.9

Number of peaks

18845 (qval < 1E-05)