Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ESR1

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MCF-7/c-Jun -Tet cells
cell line
MCF-7
cell type
ERalpha-positive breast cancer cells
chip antibody
ERalpha (H-20, Santa Cruz)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei, c-Jun and ERα complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
25387124
Reads aligned (%)
90.9
Duplicates removed (%)
7.5
Number of peaks
3464 (qval < 1E-05)

hg38

Number of total reads
25387124
Reads aligned (%)
93.4
Duplicates removed (%)
5.7
Number of peaks
3392 (qval < 1E-05)

Base call quality data from DBCLS SRA