Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
JUN

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MCF-7/c-Jun -Tet cells
cell line
MCF-7
cell type
ERalpha-positive breast cancer cells
chip antibody
c-Jun (H-79, Santa Cruz)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei, c-Jun and ERα complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
36990407
Reads aligned (%)
88.8
Duplicates removed (%)
9.8
Number of peaks
13259 (qval < 1E-05)

hg38

Number of total reads
36990407
Reads aligned (%)
91.4
Duplicates removed (%)
7.9
Number of peaks
13332 (qval < 1E-05)

Base call quality data from DBCLS SRA